Search results for "Peak area"
showing 5 items of 5 documents
Direct chromatographic study of the enantioselective biodegradation of ibuprofen and ketoprofen by an activated sludge
2018
[EN] The quantification of the enantiomeric fraction (EF) during the biodegradation process is essential for environmental risk assessment. In this paper the enantioselective biodegradation of ibuprofen, IBU, and ketoprofen, KET, two of the drugs most consumed, was evaluated. Biodegradation experiments were performed in batch mode using a minimal salts medium inoculated with an activated sludge (collected from a Valencian Waste Water Treatment Plant) and supplemented with the racemate of each compound. The inoculum activity was verified using fluoxetine as reference compound. The experimental conditions used (analyte concentration and volume of inoculum) were chosen according to OECD guidel…
Recognition and alignment of variables from UV–vis chromatograms and application to industrial enzyme digests classification
2017
Abstract In the last years, industrial applications of chemometrics have largely increased due to their capacity to extract important information from complex records as chromatograms or spectra data. The use of chemometric methods also can avoid the use of detectors of elevated cost. In this work, a procedure to recognize the relevant chemical information contained in complex UV–vis chromatograms, after a trypsin digestion, to identify the three enzyme main classes (proteases, amylases and cellulases) commonly employed in the cleaning industry, has been developed. In order to recognize the chromatogram peaks, six indices of peak identity or identifiers were defined. A program written in MA…
Ceftazidime Determination in Serum by High-pressure Liquid Chromatography
2004
A rapid and sensitive high-pressure liquid chromatographic method with simple sample preparation was developed for the quantitative analysis of the beta-lactam antibiotic ceftazidime (CAS 78439-06-2, Fortum). A good linear relationship was established between the peak area and the amount of ceftazidime injected over a concentration range of 1 to 200 microg/ml. The detection limit of the method was calculated to be 0.9 microg/ml. Stability was shown at 4 degrees C and at -196 degrees C for time periods of 2 h and 84 days, respectively.
FT-Raman determination of Mepiquat chloride in agrochemical products
2004
Abstract A fast and environmentally friendly procedure has been developed for Fourier transform Raman (FT-Raman) determination of a quaternary ammonium compound, Mepiquat chloride, in pesticide emulsifiable concentrate formulations. The method is based on the measurement of peak area values between 711 and 701 cm −1 corrected using a baseline defined at 620 cm −1 and interpolated in an external calibration of Mepiquat chloride standards dissolved in water. FT-Raman spectra were collected over 1.5 min from samples confined in screw cap standard chromatographic glass vials, at a nominal resolution of 4 cm −1 accumulating 25 scans per spectra and using a laser power of 1000 mW. Five commercial…
Monitoring of the smoking process by multicommutation Fourier Transform Infrared spectroscopy
2007
Abstract Nicotine was selected as the target molecule for monitoring of the smoking process by multicommutation Fourier Transform Infrared spectroscopy (FTIR). The method involved the use of CHCl3 for on-line extraction of nicotine from tobacco, cigarette filters and tobacco ash from NH4OH alkalinized samples, and absorbance measurement of the characteristic band at 1316 cm−1 in the stopped-flow mode, by obtaining the peak area in the range between 1334 and 1300 cm−1. Under the best operational conditions, the procedure developed provided a detection limit of 0.05 mg mL−1 nicotine, corresponding to 0.5 mg g−1 in the solid sample, a relative standard deviation less than 2.5%, and a sampling …